Automated Imaging and Analysis for the Quantification of Fluorescently Labeled MacropinosomesKoen M. O. Galenkamp 1, Cheska Marie Galapate 1, Yijuan Zhang 1, Cosimo Commisso 1
1Sanford Burnham Prebys Medical Discovery Institute, NCI-designated Cancer Center
Automated assays using multi-well microplates are advantageous approaches for identifying pathway regulators by allowing the assessment of a multitude of conditions in a single experiment. Here, we have adapted the well-established macropinosome imaging and quantification protocol to a 96-well microplate format and provide a comprehensive outline for automation using a multi-mode plate reader.
